Time (min)	Description	Detector flow (mL min−1)	Focus flow	Cross flow	Injection flow
0–2	Elution	0.5	0	0	0
2–3	Focus	0.5	1.5	0	0
3–5	Focus + injection	0.5	1.5	0	0.2
5–7	Focus	0.5	1.5	0	0
7–57	Elution	0.5	0	0.1	0
57–62	Elution	0.5	0	0	0
62–67	Elution + injection	0.5	0	0	0.2
67–69	Elution	0.5	0	0	0