| Time (min) | Description | Detector flow (mL min-1) | Focus flow | Cross flow | Injection flow |
| 0–1 | Elution | 0.5 | 0 | 0 | 0 |
| 1–11 | Elution + injection | 0.5 | 0 | 0 | 0.2 |
| 11–12 | Elution | 0.5 | 0 | 0 | 0 |
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