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. 2016 Aug 22;408(24):6857–6868. doi: 10.1007/s00216-016-9812-5

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — Overlay ion images of intact PC (32:0) [M + K]⁺ at m/z 772.52 (red) and sphingolipid C₄₈H₉₁NO₈Na [M + Na]⁺ at m/z 832.66 (green). The pixel size was set to 12 μm for image (a) (2400 × 2400 μm², with the number of counts 20,337 for m/z 772.52 and 15140 for m/z 832.66) and 3 μm for image (b) (500 × 500 μm², with the number of counts 9525 for m/z 772.52 and 9040 for m/z 832.66). The line scan graph was obtained from image (b) indicates an approximate spatial resolution of 4 μm (c)