Table 2. Mass transitions used for the MRM detection of common AHLs.
| Acyl chain length | Carbon 3 substitution | MRMs | Retention time/min |
|---|---|---|---|
| C4 | Unsubstituted | 172–102 | 3.18 |
| Oxo | 186–102 | 2.02 | |
| OH | 188–102 | 1.58 | |
| C6 | Unsubstituted | 200–102 | 4.53 |
| Oxo | 214–102 | 4.08 | |
| OH | 216–102 | 3.93 | |
| C8 | Unsubstituted | 228–102 | 5.20 |
| Oxo | 242–102 | 4.66 | |
| OH | 244–102 | 4.49 | |
| C10 | Unsubstituted | 256–102 | 5.87 |
| Oxo | 270–102 | 5.31 | |
| OH | 272–102 | 5.05 | |
| C12 | Unsubstituted | 284–102 | 6.65 |
| Oxo | 298–102 | 5.98 | |
| OH | 300–102 | 5.72 | |
| C14 | Unsubstituted | 312–102 | 7.44 |
| Oxo | 326–102 | 6.77 | |
| OH | 328–102 | 6.50 |