Table 1. Wild type and GFP expressing cell lines of P. tricornutum used for time-dependent GFP-fluorescence measurements by flow cytometry and fluorescence microscopy.

Protein IDs refer to wild type strain CCMP632 (denoted “Pt1” by Martino et al. (2007)), which was sequenced by the U.S. Department of Energy Joint Genome Institute (http://genome.jgi.doe.gov/Phatr2/Phatr2.home.html) (Bowler et al., 2008).

Cell line name	Description
Wt	Wild type Phaeodactylum tricornutum Bohlin (University of Texas Culture Collection, Austin, strain UTEX646) (denoted “Pt4” by Martino et al. (2007))
lhcf1-GFP	P. triconutum UTEX646, genetically transformed with the pPha-T1-GFP plasmid (Gruber et al., 2007), derived from pPha-T1 (GenBank AF219942.1, Zaslavskaia et al. (2000)), which contains the 442 bp 5′-flanking region of the P. triconutum lhcf1 gene (equivalent protein in Pt1, ID 18049) (Apt, Kroth-Pancic & Grossman, 1996) as promoter
nr-GFP_3	P. triconutum UTEX646, genetically transformed cell lines expressing the eGFP gene with a construct derived from the pPha-NR vector (GenBank JN180663.1, Stork et al. (2012)), which contains the 422 bp 5′-flanking region of the P. triconutum nr gene (equivalent protein in Pt1, ID 54983) (Hempel et al., 2009) as promoter (see text for details)
nr-GFP_4
nr-GFP_5
nr-GFP_6
nr-GFP_9
nr-GFP_10