. 2016 Jul 6;204(1):139–152. doi: 10.1534/genetics.116.191056

Table 2. Chi alleles and their nucleotide sequence contexts.

Chi allele	Sequence (5′ → 3′)^a	Description (refs)^b
χ⁺76^c	GGCGAGCTGCTGGTGGTGACGCGCCC	Chi⁺ mutant of λ (Stahl and Stahl 1977; Kobayashi et al. 1982)
χ⁺D123	TCGTGAAAGCTGGTGGCAGGAGGTCG	Chi⁺ mutant of λ (Smith et al. 1981a)
χ⁺C157	GCAGATCAGCTGGTGGAAGAGGGACT	Chi⁺ mutant of λ (Sprague et al. 1978)
χ⁻C206	GCAGATCAGTTGGTGGAAGAGGGACT	Octamer mutation of χ⁺C157 (Schultz et al. 1981)
χ⁺F225	CCGGCTAGGCTGGTGGGGTTGCCTTA	Chi⁺ mutant of λ (Smith et al. 1981b)
χ⁺G218	AAACCACCGCTGGTGGCGGTGGTTTT	Chi⁺ mutant of λ (Smith et al. 1981b)
χ⁺J270	TGGGTGTGCTCTGCTGGTGGGCGGG	Random 25-bp insertion in gam
χ⁺J271	TGGTTGTTTTTGGCTGGTGGGTGGT	“
χ⁺J272	TGGGGTGTGCTCTGCTGGTGGGCGG	“
χ⁺J273	GATCGGGTCAGGGGAGAGCTGGTGG	“
χ⁺J274	GGAGGTGCTGGTGGCCTTTGGTACG	“
χ⁺J275	CACCAGCTGGTGGTCCTAGGTGTTC	“
χ⁺J276	GCTGGTGGAGGCAGGTAATGCCATT	“
χ⁺L251	TTAGTGATGCTGGTGGTTGATCTCA	Chi octamer in gam; wt flanks
χ⁻L251	TTAGTGATGTTGGTGGTTGATCTCA	Octamer mutation of χ⁺L251
χ⁺L252	TTAGCCATGCTGGTGGACGGTCTCA	Chi octamer in gam; mutant flanks
χ⁻L252	TTAGCCATGTTGGTGGACGGTCTCA	Octamer mutation of χ⁺L252
χ⁺L253	TTAGCAAAGCTGGTGGCGACTCTCA	Chi octamer in gam; mutant flanks
χ⁺L254	TTAGCATAGCTGGTGGAGACTCTCA	Chi octamer in gam; mutant flanks
χ⁺L255 (2-3)	TTAGCCATGCTGGTGGCGACTCTCA	Swap of 5′ and 3′ flanks
χ⁺L256 (2-4)	TTAGCCATGCTGGTGGAGACTCTCA	“
χ⁺L257 (3-2)	TTAGCAAAGCTGGTGGACGGTCTCA	“
χ⁺L258 (4-2)	TTAGCATAGCTGGTGGACGGTCTCA	“
χ⁺L259	TTAGTGATGCTGGTGGTCGAAAACA	“Hottest” flank cut by RecBCD
χ⁻L259	TTAGTGATGTTGGTGGTCGAAAACA	Octamer mutation of χ⁺L259
χ⁺L262	TTAGTGATGCTGGTGGGGGCTTCCA	“Colder” flank cut by RecBCD

refs, references; wt, wild type.

Sequence surrounding the Chi octamer (underlined) in the indicated allele in λ. The χ⁻ octamer mutation (C → T) is in boldface type.

See Materials and Methods for sources of nonreferenced alleles.

χ⁺76 is A → G mutation at nucleotide 33,058 in the gam gene, changing gctggtAg to gctggtGg (unpublished data).