Figure 7.

The catalytic domain of Bni1 is not sufficient to provide essential formin functions in the absence of Dma1/2. (A) Schematic representation of Bni1 indicating its relevant regulatory domains and the constructs used in B (Chen et al. 2012). All constructs were tagged at the N terminus with three HA and one GFP. GBD, GTPase-binding domain; SBD, Spa2-binding domain; BBD, Bud6-binding domain; DID, diaphanous inhibitory domain; DAD, diaphanous autoregulatory domain; FH1–FH3, formin homology 1–3. (B) Serial dilutions of strains with the indicated genotypes were spotted on YEPD plates and incubated at the indicated temperatures.