Table 2. Reciprocal crosses between third chromosome homokaryotypic D. pseudoobscura strains to generate heterokaryotypes.

ID	Male homokaryotype	Female homokaryotype
Cross 1A	AR KB945	ST MSH49
Cross 1B	ST MSH49	AR KB945
Cross 2A	AR KB945	CH MSH202
Cross 2B	CH MSH202	AR KB945
Cross 3A	ST MSH49	CH MSH202
Cross 3B	CH MSH202	ST MSH49
Cross 4A	AR KB945	PP DM1054
Cross 4B	PP DM1054	AR KB945
Cross 5A	AR KB945	AR DM1005
Cross 5B	AR DM1005	AR KB945

Each cross was replicated three times for RNA-seq.