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. 2016 Jun 28;204(1):327–336. doi: 10.1534/genetics.115.185314

Figure 3.

Figure 3

The effect of RNAi-mediated inhibition of 71 genes from WGA mapping in RAW264.7 macrophages on LTA-induced IL-6 production. Pools of four siRNA duplexes per gene were transfected into RAW264.7 cells, LTA was added, and cytokine production was monitored. Shown are the results for negative control siRNA (blue; nontargeting siRNA from Dharmacon), four positive control siRNAs (red; Traf6, Tlr6, Il-6, and Map3k7), and 71 siRNAs targeting genes identified by our eQTL mapping analysis (black). All data are normalized to the NT#1 nontargeting negative control siRNA. Means of four independent measurements with error bars representing SEMs are plotted; green line indicates threshold for twofold reduction in IL-6 response as a result of gene inhibition. *P < 0.0001, #P < 0.001, and ^P < 0.01 by one-group two-tailed t-test testing for deviation from 1.