Figure 5.

Effect of overexpression of four target genes in RAW264.7 cells on NF-κB activation. Plasmids containing full-length cDNAs for the indicated genes (or CAT negative control) cloned downstream of the CMV promoter were cotransfected with NF-kB-AP1-luc and SV40-rluc plasmids. Following transfection, cells were stimulated with 2.5 µg/ml LTA for 5 hr, and innate immune responsiveness was monitored by measuring luciferase production. Means of three independent measurements with error bars representing SEMs are plotted, and red line indicates threshold for twofold increase in IL-6 response as a result of gene overexpression. Compared to CAT (set to 1), ^P < 0.01 by unpaired two-group two-tailed t-test.