Table 1. List of published DMS studies assessing mutant growth rates in accordance with our statistical model, arranged by number of time points sampled.

Reference	No. of time points	No. of mutants	Total no. of sequence reads (millions)	Reproducibility (if reported)	Summary of reference
Hietpas et al. (2013)	6 or 7	568	30	$R^2=0.95$ for full replicates	Beneficial single substitutions in Hsp90 of yeast under altered environments
Jiang et al. (2013)	6 or 7	568	34	$R^2=0.96$ for full replicates	Interaction of expression and single substitutions on DFE of yeast Hsp90
Puchta et al. (2016)	5–7	≈60,000	545.7	$R^2=0.85$	Network of epistatic interactions in yeast small nucleolar RNA
Roscoe et al. (2013)	6	1,530	21	$R^2=0.93$ for full replicates	Functional biophysics of single substitutions in ubiquitin of yeast
Hietpas et al. (2011)	3 or 7	568	26	$R^2=0.82$ for full replicates	DFE of single substitutions for a short region of Hsp90 in yeast
Bank et al. (2015)	5	1,015	21.6	Credibility intervals (figure 6B)	DFE of epistatic substitutions in Hsp90 of yeast
Wu et al. (2013)	3	>400	>0.002	NA	Compensatory single substitutions for neuraminidase mutant of influenza
Li et al. (2016)	2	65,537	685.5	$R^2=0.997$ mean across 15 pairs of biological replicates	Fitness landscape of a transfer RNA gene in yeast
Jiang et al. (2015)	2	475	20.5	$R^2=0.92$ for full replicates	Biophysics of single substitutions in influenza neuraminidase with antiviral
Roscoe and Bolon (2014)	2	1,617	30	$R^2=0.96$ for full replicates	Biophysics of single ubiquitin substitutions on E1 activity and yeast fitness
Melnikov et al. (2014)	2	4,993	33.2	$R^2=0.90$ for full replicates	Biophysics of single substitutions in APH(3′)II in Escherichia coli with antibiotics
Kim et al. (2013)	2	29,708	90.2	NA	Biophysics of single substitutions in yeast Deg1 protein degradation signal
Melamed et al. (2013)	2	110,745	186.5	NA	Biophysics of single and multiple substitutions in Pab1 RRM of yeast
Klesmith et al. (2015)	2	9,219	5.8	Reproducibility plot	Biophysics of levoglucosan consumption rate in E. coli