Table 3. Characteristics of the 34 microsatellite markers developed in Lutjanus johnii, Protonibea diacanthus and Lethrinus laticaudis.
| Species | Locus | Primer sequences (5′–3′) (fluorescent label) | Repeat motif | GenBank accession no. | n | Allele size range (bp) | #A | HO | HE | PHW | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Lutjanus johnii | Luj027 | F: | CTGGGCCACACTGATAAAGC (FAM) | (AGC)9 | KX387441 | 68 | 152–179 | 8 | 0.309 | 0.306 | 0.606 |
| R: | GGCTCTGAACCTGGGAGATT | ||||||||||
| Luj094 | F: | TCTCAGAGGGTTTGATGCAG (FAM) | (AATC)9 | KX387437 | 68 | 223–239 | 4 | 0.426 | 0.470 | 0.205 | |
| R: | CTTTGGCGCTTTCTATCAGC | ||||||||||
| Luj076 | F: | CGGGTCGAGTCTGTTTGTGT (VIC) | (AAG)15 | KX387436 | 66 | 200–233 | 10 | 0.818 | 0.811 | 0.289 | |
| R: | CTTCAGACGGATTAGCAGCA | ||||||||||
| Luj068 | F: | CCTAGGGTGTCAGTGTCAGTCA (PET) | (AAAG)20 | KX387435 | 68 | 174–258 | 18 | 0.882 | 0.936 | 0.212 | |
| R: | TGCCTGTATGTTCTCTTGAGC | ||||||||||
| Luj090 | F: | ATCCTAATGCATCGTGCTTG (FAM) | (AGC)17 | KX387444 | 68 | 194–278 | 23 | 0.868 | 0.922 | 0.146 | |
| R: | GGCATGTTCTATTGAGGTTGG | ||||||||||
| Luj051 | F: | TGCAGAGCAACAGAACAACAC (VIC) | (ACTG)10 | KX387440 | 67 | 172–192 | 6 | 0.687 | 0.596 | 0.377 | |
| R: | CACCTTGCGTTTGCAGTCT | ||||||||||
| Luj114 | F: | CCATAACTGCTGTTCTGTATCTGG (VIC) | (AGC)9 | KX387442 | 68 | 276–314 | 11 | 0.794 | 0.739 | 0.725 | |
| R: | AATACGGCAGATCTCGGGTT | ||||||||||
| Luj091 | F: | TCATTCCCAGGAGCTCAAAT (PET) | (ACAG)12 | KX387438 | 64 | 219–279 | 12 | 0.813 | 0.782 | 0.499 | |
| R: | AATCGTCACTTTCGACCCAC | ||||||||||
| Luj072 | F: | ACTCGAAGAACACAGCCCAC (FAM) | (AGC)9 | KX387443 | 65 | 192–204 | 5 | 0.738 | 0.675 | 0.052 | |
| R: | CACATTTGAATCCTTGCTGG | ||||||||||
| Luj082 | F: | AAGTACATCGGAGGGCTGAG (VIC) | (ACGAT)12 | KX387439 | 65 | 220–275 | 12 | 0.800 | 0.833 | 0.548 | |
| R: | TGTTATCAAAGTTCACCGATACAAA | ||||||||||
| Protonibea diacanthus | Prd044 | F: | ACAAAGTTTCCTCCTCTGGC (FAM) | (AAG)13 | KX387452 | 71 | 181–211 | 11 | 0.746 | 0.804 | 0.324 |
| R: | CACGTTCCATCTTTATTTATTTGC | ||||||||||
| Prd023 | F: | TCGTGTGAACACTTTGATGC (VIC) | (ATC)11 | KX387448 | 72 | 292–316 | 9 | 0.875 | 0.847 | 0.034 | |
| R: | CTCGTCTCTGCTCTTGGTCC | ||||||||||
| Prd042 | F: | TACCTTTGAGATGCGAGCG (NED) | (AGC)12 | KX387451 | 72 | 230–248 | 7 | 0.694 | 0.698 | 0.526 | |
| R: | GTCAAAGCCATCAATCCAGC | ||||||||||
| Prd012 | F: | AGGCTGTTTGAACTGCAGGG (PET) | (AAAG)20 | KX387445 | 64 | 195–271 | 19 | 0.828 | 0.898 | 0.324 | |
| R: | CATGCTGAGCAATATGTGGG | ||||||||||
| Prd046 | F: | TCATCCTGAGTTTGTGCTGG (FAM) | (AGC)9 | KX387454 | 72 | 224–236 | 5 | 0.347 | 0.351 | 0.693 | |
| R: | CATGAGTAAGCAGAGCGTGG | ||||||||||
| Prd018 | F: | ATGAACGGCATCAGTCAGC (NED) | (ACAG)9 | KX387446 | 68 | 179–207 | 8 | 0.662 | 0.784 | 0.008 | |
| R: | CGTCTGATAAACAGCACTGCC | ||||||||||
| Prd020 | F: | CAATGTTCTGCAAGAGCTGC (PET) | (ATC)11 | KX387447 | 71 | 189–216 | 10 | 0.746 | 0.713 | 0.837 | |
| R: | TCAAATGTCAAAGTCCAGTCC | ||||||||||
| Prd045 | F: | GTCTATCCATGTTCCAGCCC (PET) | (ATCC)11 | KX387453 | 64 | 279–309 | 8 | 0.672 | 0.639 | 0.400 | |
| R: | TCATCCCAAAGTGACCAACC | ||||||||||
| Prd049 | F: | CCTTGTCCTCCTTTCAGGC (FAM) | (ACC)9 | KX387455 | 72 | 216–234 | 5 | 0.278 | 0.316 | 0.280 | |
| R: | GGGTCATTAAACATGGCAGC | ||||||||||
| Prd036 | F: | TCACGTGAAGCGTCTACAGC (VIC) | (AAG)12 | KX387450 | 72 | 227–257 | 9 | 0.653 | 0.677 | 0.746 | |
| R: | AAAGGAGGAAACACAGAGCC | ||||||||||
| Prd024 | F: | AGAGTGTCCGAGTCCAGAGG (PET) | (AAG)11 | KX387449 | 72 | 199–217 | 6 | 0.736 | 0.683 | 0.892 | |
| R: | CAGTACCTGGTGATGGGAGC | ||||||||||
| Lethrinus laticaudis | Lel011 | F: | CTGTCGGAGGTAAAGTGCG (FAM) | (AGC)9 | KX387422 | 84 | 237-285 | 11 | 0.655 | 0.660 | 0.251 |
| R: | CTCATGGTGTTGAGGATGGG | ||||||||||
| Lel040 | F: | TGGTTGCAGACAACTGCC (VIC) | (AGC)9 | KX387431 | 84 | 171–216 | 14 | 0.821 | 0.825 | 0.367 | |
| R: | CTTAAGAGCAGTGATCCAGGC | ||||||||||
| Lel033 | F: | AGTGCGACAAAGAAATGGC (NED) | (AGAT)16 | KX387428 | 84 | 167–143 | 20 | 0.893 | 0.926 | 0.438 | |
| R: | CATTTGTCAGTTATGAAACTTGGC | ||||||||||
| Lel012 | F: | GCGAGGGTCTGCTACTATAGGG (PET) | (AAT)9 | KX387423 | 76 | 246-334 | 22 | 0.711 | 0.846 | 0.005 | |
| R: | TGTAAAGTGTAAACCACGTCCC | ||||||||||
| Lel013 | F: | CCTGAACCTGGAGAACTCGG (FAM) | (ATC)12 | KX387424 | 82 | 242–287 | 10 | 0.744 | 0.829 | 0.150 | |
| R: | ACTGAGGGAGGAGATAAAGGG | ||||||||||
| Lel041 | F: | CTGCTGTTCTGGGTTGCC (VIC) | (AAT)19 | KX387432 | – | – | – | – | – | – | |
| R: | CAACAAGCTGTTGGTGTCCC | ||||||||||
| Lel032 | F: | AAATCTGCATTATGAAATTGGC (NED) | (AAAG)16 | KX387427 | 83 | 173–233 | 16 | 0.867 | 0.883 | 0.209 | |
| R: | CAGCTCCTTGAGTTTAGTCCC | ||||||||||
| Lel028 | F: | CAGTAGCTTTAATAGTTAGGCACCC (PET) | (AAAG)13 | KX387426 | 83 | 200–244 | 14 | 0.843 | 0.877 | 0.954 | |
| R: | GGCTGTCCAGAGTGAGGC | ||||||||||
| Lel047 | F: | AAAGAATGGGAAGAATGACCC (FAM) | (AGAT)11 | KX387434 | – | – | – | – | – | – | |
| R: | AAGCCAAGTGATTAAGAAACCC | ||||||||||
| Lel027 | F: | CACTAAGGGTCCATGTTGCC (FAM) | (AAT)22 | KX387425 | 79 | 196–238 | 15 | 0.911 | 0.914 | 0.480 | |
| R: | TCTGTAATGAATGATCAAACCG | ||||||||||
| Lel044 | F: | TTCTACTTGACCCTGGTAGGC (VIC) | (ATCC)11 | KX387433 | 83 | 151–199 | 11 | 0.759 | 0.816 | 0.268 | |
| R: | AATGTAATGCCATAAGCGGG | ||||||||||
| Lel036 | F: | TCCAATTTACACCAAACTAGGC (NED) | (AAAG)15 | KX387429 | – | – | – | – | – | – | |
| R: | CCGGAATGATCTGCAGGC | ||||||||||
| Lel039 | F: | CTTGTAGAGTGTCAACGAGGG (PET) | (AAT)11 | KX387430 | 80 | 196–214 | 9 | 0.700 | 0.764 | 0.747 | |
| R: | CATGATGCAATAACCATCCC | ||||||||||
Notes.
n is the sample size, #A is the number of alleles at each loci, HE is the expected heterozygosity, HO is the observed heterozygosity and PHW is the p-value of the exact tests of conformance of genotypic proportions to Hardy-Weinberg equilibrium expectations.