Supplemental Figure S2.

Mimics of miR-185 and miR-186 alleviate transforming growth factor (TGF)-β–induced epithelial-mesenchymal transition (EMT) in HCC827 cells. HCC827 cells were transfected with miR-185 mimic, miR-186 mimic, or nontargeting control miRNA 6 hours before TGF-β induction. A: Western blotting using anti–E-cadherin and anti–α-smooth muscle actin (SMA) antibodies was performed after 24 hours of TGF-β induction. B: The density ratio of E-cadherin to α-SMA of each specimen was calculated after densitometry analysis. The results are from the same experiment as Supplemental Figure S1, and the Western blot shown is representative of three independent experiments. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.