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. 2016 Sep;186(9):2310–2316. doi: 10.1016/j.ajpath.2016.04.015

Figure 4.

Figure 4

Mimics of miR-185 and miR-186 block transforming growth factor (TGF)-β–induced type 5 collagen [Col(V)] overexpression in A549 cells. A549 cells were transfected with miR-185 mimic, miR-186 mimic, or nontargeting control miRNA 6 hours before TGF-β induction. Western blotting using anti-Col(V) and anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies was performed after 24 hours of TGF-β induction. A: Representative image of three independent experiments. B: Densitometry analysis of Col(V)/GAPDH. ∗P < 0.05 versus control.