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. 2016 Sep;186(9):2500–2514. doi: 10.1016/j.ajpath.2016.05.016

Figure 2.

Figure 2

PDK4 increases in pulmonary arterial hypertension (PAH) pericytes. A: Confocal images of PDK4 lung sections. Endothelium was stained with CD31 (green), pericytes with 3G5 (red), and lung tissue with PDK4 (white). White arrows indicate pericyte-positive staining, and yellow arrows indicate co-staining of 3G5 and PDK4. DAPI labels cell nuclei (blue). B: TaqMan quantitative PCR for the four PDK family proteins in donor control and PAH pericytes. The expression of each gene is shown relative to that in donor control pericytes. C: Representative Western immunoblot images of PDK4 in enriched mitochondria of pericytes from three donor control (Ctrl) versus three patients with PAH. ∗∗∗P < 0.001 versus unstimulated controls using the unpaired t-test. Scale bar = 50 μm. OD, optical density.