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. 2016 Sep;186(9):2500–2514. doi: 10.1016/j.ajpath.2016.05.016

Figure 4.

Figure 4

Increased PDK4 expression correlates with reduced mitochondrial activity in pulmonary arterial hypertension (PAH) pericytes. A: Reactive oxygen species (ROS) production in PAH versus donor control (Ctrl) cells. Fluorescence intensity (rel. Fluor.) was normalized against MitoTracker (MitoT). B: Fluorescence activated cell sorting of healthy donor and PAH pericytes using mitochondrial membrane potential (MMP). FL1-A: fluorescein isothiocyanate; FL3-A: Texas Red. The percentage of high MMP is found in the block of Q2-UR. C: Aerobic glycolysis and glycolytic capacity in cells as measured by Seahorse analysis. ∗∗P < 0.01, ∗∗∗P < 0.001 compared with controls using unpaired t-test. Scale bar = 50 μm. CCCP, Carbonyl cyanide 3-chlorophenylhydrazone; ECAR, extracellular acidification rate; LL, lower left; LR, lower right; mpH, mili pH per minute; UL, up-left; UR, up-right.