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. 2016 Sep;186(9):2271–2278. doi: 10.1016/j.ajpath.2016.05.020

Figure 2.

Figure 2

Decreased cell survival and increased DNA damage levels after knockdown (KD) of MSI1. A: Analysis of MSI1 expression in U251 cells transfected with control (shCtrl) or shMSI1 by Western blot. B: Clonogenic assay of U251 MSI1-KD after exposure to X-rays. C: DNA damage and repair assessed by the alkaline comet assay at different times after radiation. D: Representative field with U251 cells with 53BP1 foci after IR treatment. E: Quantification of 53BP1 ionizing radiation–induced foci (IRIF) levels in U251 cells after MSI1 knockdown. Data are presented as means ± SD. Data was collected from 3 sets of 100 cells each per condition. ∗∗P < 0.01, ∗∗∗P < 0.001 (t-test). Scale bars = 25 μm. NT, no treatment; sh, small hairpin; si, small interfering.