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. 2016 Sep;186(9):2429–2448. doi: 10.1016/j.ajpath.2016.05.021

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© 2016 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

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Immunostaining for dystrophin and laminin α2 surrogates in GALGT2-overexpressing FKRP P448Lneo⁻ muscle. FKRP P448Lneo⁻ muscles (gastroc) were injected with rAAVrh74.MCK.GALGT2 and analyzed at 3 months after injection. Cross-sections of muscle were immunostained with antibodies to the indicated proteins (red) and counterstained with WFA (green). Nuclei are stained with Hoechst 33342 (blue). Scale bar = 50 μm. GALGT2, β-1,4 N-acetylgalactosaminyltransferase 2; gastroc, gastrocnemius; MCK, muscle creatine kinase; rAAV, recombinant adeno-associated virus; WFA, Wisteria floribunda agglutinin.