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. 2016 Sep;186(9):2429–2448. doi: 10.1016/j.ajpath.2016.05.021

Figure 5.

Figure 5

Quantification of immunoglobulin G (IgG) uptake after overexpression of GALGT2 in FKRP P448Lneo− mice. The gastroc, quad, and TA muscles of FKRP P448Lneo− mutant mice were injected with rAAVrh74.MCK.GALGT2 (treated) or PBS (untreated) and compared at 1, 3, and 6 months after injection. A: Gastroc muscle cross-sections were stained with WFA (green) to label CT glycan overexpression and an anti-mouse IgG antibody to identify myofibers with IgG uptake (red). Nuclei were stained with DAPI (blue). B: Quantification of the percentage of total IgG+ area in muscle cross-sections. Error bars represent SEM. n = 15 measurements from three muscles per group. *P < 0.05, **P < 0.01, and ***P < 0.001. Scale bar = 100 μm. CT, cytotoxic T cell; GALGT2, β-1,4 N-acetylgalactosaminyltransferase 2; gastroc, gastrocnemius; MCK, muscle creatine kinase; PBS, phosphate-buffered saline; quad, quadriceps; rAAV, recombinant adeno-associated virus; TA, tibialis anterior; WFA, Wisteria floribunda agglutinin.