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. 2016 Jul 7;36(9):1579–1591. doi: 10.1177/0271678X16657836

Figure 2.

Figure 2.

Retinal ganglion cell loss following vessel occlusion. (a–c) Photothrombosis of a single venule produced large-scale loss of retinal ganglion cells (RGCs) in the region supplied by the occluded vessel following a five-day survival time. Immunohistochemical labeling of the RGC marker Brn3a (a) reveals a complete absence of RGCs in the region supplied by the occluded vessel (yellow dot). Image retouched to remove dust artifacts. (b) A pseudocolor map of the density of Brn3a-positive RGCs in the retina in A, showing a decrease in RGC density in the region supplied by the occluded vessel. (c) RGC density measured along a circle 2.0 mm from the optic disc (dotted line in B). Brn3a-positive RGCs are completely absent close to the occluded vessel, but are present in high numbers in non-ischemic regions of the retina. (d–e) Differential interference contrast microscopy of a different preparation following a 14-day survival time demonstrates an absence of cells in an ischemic region (d), compared to a healthy region far from the thrombus (e).V, vessel.