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. Author manuscript; available in PMC: 2017 Sep 2.
Published in final edited form as: Virus Res. 2016 Jul 25;223:161–169. doi: 10.1016/j.virusres.2016.07.009

Figure 1.

Figure 1

Replication of HIV-1 in Jurkat and WB analysis of viral proteins. (A) HIV-1 corresponding to 10,000 cpm/ml was infected into Jurkat cells (1×106 cells), and replication kinetics of HIV-1 was determined by measuring RT activity in the culture supernatants every 3 days. (B) HIV-1 was infected into Jurkat cells, and cell (left) and virus (right) lysates were prepared at peak virus replication. 1/5 and 1/2 of total lysates of cells and virus (supernatants), respectively, were employed for the analysis. Band intensity of p24 (arrow) in the supernatant was approximately 1/3 of that in the cells.