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. Author manuscript; available in PMC: 2017 Sep 2.
Published in final edited form as: Virus Res. 2016 Jul 25;223:161–169. doi: 10.1016/j.virusres.2016.07.009

Figure 3.

Figure 3

Effect of CHX. USP15- and/or Nef-expressing plasmids were transfected into 293T cells, and at forty-eight hours pos-transfection, the transfected cells were treated with CHX for the indicated time. Changes in the amount of USP15 and Nef were then determined in the cells transfected with USP15- (left), Nef- (middle), or USP15- and Nef-expressing plasmids (right) by WB analysis (above) followed by quantification based on the amount of β-actin. The data were representative of three independent experiments.