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Schematic of gene silencing using an intelligent shRNA expression device (iRed). Step1: A region of plasmid DNA (pDNA) encoding the U6 promoter and a short hairpin RNA (shRNA) was amplified using PCR in the presence of one type of 2'-deoxy-4'-thionucleoside triphosphate (dSNTP) and three other dNTPs to synthesize an iRed. Step 2: The iRed was delivered into the nucleus. Step 3: Numerous shRNAs were transcribed from the iRed to possess a potent RNA interference (RNAi) activity.
