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. 2016 Jan 5;5(1):e276. doi: 10.1038/mtna.2015.49

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Development of MMP13-expressing plasmid. (a) The MMP13 expression vector, containing CAG promoter-MMP13-IRES-tdTomato-polyA cassette, was generated through a multistep and ligation-based cloning (pBGI-MMP13). (b) The expression efficiency of MMP13 from the pBGI-MMP13 was confirmed in vitro by western blotting. Control, standard recombinant MMP13 protein.