Figure 2.

Surveyor assay for gRNA screening in 293T cells and in myoblasts. The assay was performed on genomic DNA extracted from (a and b) 293T cells or (c and d) myoblasts transfected individually with different gRNAs. Screening was performed separately for (a and c) exon 50 and (b and d) exon 54. Genomic DNA of nontransfected cells was used for negative control (NC) for the Surveyor assay. The gRNA numbers correspond with the PAM identified in Figure 1a and the targeted sequences in Table 1. MW, molecular weight marker.