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gRNA pairs can induce deletions that restore the reading frame in the DMD gene in DMD myoblasts. (a) Sequence obtained from the amplification of the hybrid exon 50–54 following transfection of the gRNA2–50 and gRNA2–54 pair shows a newly formed codon TAT (coding for tyrosine) at the junction site. This new codon is formed by the nucleotide T from the remaining exon 50 and nucleotides AT from the remaining exon 54. (b) Other in-frame and out-of-frame sequences were also found.
