Fig 1. Schematic diagram of the experimental setup, including an SD-OCT system and a perfusion unit.

The perfusion unit can be used to induce pressure transients. A single reservoir can control static pressures in the cannula inserted into Schlemm’s canal (SC), while switching between two reservoirs provides a means of examining dynamic changes. A radial limbal segment is placed with the trabecular meshwork facing upward toward the OCT imaging beam and a cannula is inserted into SC.