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. 2016 Sep 6;11(9):e0161695. doi: 10.1371/journal.pone.0161695

Fig 4. Promoter activity analysis of the GhAO1 promoter and 5’-deletion constructs.

Fig 4

Different constructs were transformed into tobacco, and GUS activity was assayed. (A) Schematic presentation of the 5’-deletion constructs. The full-length and truncated fragments were fused to the GFP-GUS gene, generating the constructs P-1920, P-1760, P-1600, P-1320, P-1040, P-720, and P-360. (B) Quantitative analysis of the GUS activity of the constructs. The promoter activity was determined in transgenic tobacco leaves transformed with the different constructs. The specific GUS activity was determined as the rate of 4-methylumbelliferyl β-D-glucuronide conversion to 4-methylumbelliferone (pmol mg protein-1 min-1). The data are presented as the average of three independent experiments.