Fig 1. ASM overexpression impaired social preference (A) without altering preference for social novelty (B) or social memory (C) in female, but not in male, mice.

Percentage investigation of the empty cage, the same and the novel mouse/juvenile during the first (A) and second (B) 5 min of the social approach test (n = 10–13 per group) and during the discrimination period of the social discrimination test (C; n = 6 per group). Mice were treated with amitriptyline (black bars) or water (grey bars) for 4 weeks before and during behavioral testing. Social preference is reflected by higher investigation time directed towards the same mouse versus the empty cage (A). Preference for social novelty (B) and social discrimination (C), as indicators of intact social memory, are reflected by higher investigation time directed towards the novel versus the same mouse/juvenile. Shown are means + SEM. * p<0.05 versus empty cage (A) or same mouse (B)/juvenile (C).