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. 2016 Sep 6;11(9):e0162094. doi: 10.1371/journal.pone.0162094

Fig 8. miRNA-149-3p modulates AAMP expression by miRNA-mRNA physical interaction.

Fig 8

The expression level of AAMP in A549 cells either treated or untreated with the heptapeptide angiotensin-(1–7), or transfected with miRNA-149-3p mimic or inhibitor are presented. In addition, the effect of AAMP siRNA were also investigated. A) Relative mRNA level of AAMP in different groups of cell investigated. B) Protein extracts from different groups of cells were analyzed using Western blots and specific antibodies. β-actin was used as a loading control. C) Schematic representation of AAMP and AAMP mutated mRNA target site and the miRNA-149-3p. D) Relative luciferase activity in cells transfected with pGL3 plasmids (Control, AAMP-3’-UTR and AAMPmut-3’-UTR) and miRNA-149-3p mimics or inhibitor; the results were plotted in representative graphs. ANOVA analysis found significant differences between the control and cell samples; the significance level was set at p <0.05 (***).