Table 2. Blockade of P. berghei transmission in transgenic mosquitoes.
| Vectorial competence a (7 mosquito feedings b ) | No. of infected/total c (% infected mice) | |
|---|---|---|
| WT | AAPP-mBax Line 1 | |
| Exp 1 (day 22) d | 3/4 (75) | 0/3 (0) |
| Exp 2 (day 20) d | 5/5 (100) | 0/5 (0) |
| Total | 8/9 (88.9) | 0/8 (0) |
aVectorial competence is an evaluation of a vector’s ability to transmit a parasite to naïve mice. Wild-type (WT) mosquitoes and AAPP-mBax line 1 mosquitoes (homozygous) were allowed to feed on the same P. berghei-infected mouse for 30 min, and fully engorged mosquitoes were collected. Seven mosquitoes were fed on individual naïve mice on day 22 (Exp 1) or day 20 (Exp 2) after the infectious blood meal for more than 30 min. The infection status of each mouse was evaluated by a microscopic examination of a Giemsa-stained blood smear on days 4, 7, 10, and 17 after mosquito feeding. Mice that had no parasites by day 30 were defined to be uninfected.
b, cThe infection status of each mouse and mosquito is shown in S2A and S2B Table.
dTwo independent experiments were performed using separate generations of mosquitoes.