Fig 3. One copy gene responses in different Df/+ settings.

A-D) One copy versus two copy gene expression levels plotted at the centers of each gene model (labeled below). Observations made in the isogenic genetic background (filled) and the hybrid genetic background (open) are shown. Genes whose expression is below the expression cutoff (see Methods) are not shown. A, B) One copy gene expression of the genes between chr2L:12,545,800 and 12,975,028, which is uncovered by five different Dfs. C, D) One copy gene expression of the genes between chr2L:19,003,398 and 19,158,447 region that is uncovered by four different Dfs. E, F) Sashimi plots that display normalized numbers of mapped reads across RpL30 and RpL7-like gene body regions. Expression in Df(2L)ED1202/+ for RpL30 and Df(2L)ED761/+ for RpL7-like was compared to expression in Df(2L)ED774/+, which was the shortest deletion in our study and is +/+ for both genes. Exons (black bars) in the gene models and transcription direction (chevrons) are show below. G, H) Dosage responses (y-axis) of one copy genes when uncovered by deletions of indicated length (x-axis). Results from the isogenic genetic background (top) and from the hybrid genetic background (bottom) are shown.