Table 1.
Target | Sequences of primers (5′ to 3′) | Primer size (bp) |
---|---|---|
CYP450 1A2 | GACACGGTGATTGGCAGAGAT | 115 |
TGGTGAAGGGGACAAAGGAT | ||
CYP450 2C11 | AATCCGCAGTCTGAGTTTACCC | 175 |
GGTTTCTGCCAATTACACGTTCT | ||
CYP450 3A2 | ATAAAGCCCTGTCTGATGTTGAA | 194 |
CATCACAATATCGTAGGTAGGAGGT | ||
CAR | CCTTTTCCGTTCCCTGACCA | 150 |
ACCGCATCTTCCATCTTGTAGC | ||
PXR | GCAGGGGCTGACAGAAGAAC | 200 |
CACTGTCTTTCATGATTTGACTCCA | ||
GAPDH | CCTTCCGTGTTCCTACCCC | 131 |
GCCCAGGATGCCCTTTAGTG |
Abbreviations: PCR, polymerase chain reaction; CYP, cytochrome; CAR, constitutive androstane receptor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PXR, pregnane X receptor.