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. 2016 Sep 6;11(9):e0162000. doi: 10.1371/journal.pone.0162000

Fig 6. Functional assay of S100A12 with the RAGE V domain and tranilast.

Fig 6

(a) SW480 cells were treated with 0 nM (control), 10 nM, 50 nM, or 100 nM S100A12. Cell proliferation was determined after the SW480 cells had been starved of serum for 24 h (lanes 1–4) by adding the WST-1 agent and measuring the optical density. The effects of the other treatments (S100A12 plus 1 μM RAGE V domain and S100A12 plus 1 μM tranilast) on cell proliferation activity were measured for a further 48 h (lanes 5–6). Neither tranilast nor RAGE V domain alone had an effect on cell proliferation activity (lanes 7–8). (b) The serum-starved SW480 cells were treated with 100 nM S100A12, 100 nM S100A12 plus 1 μM FPS-ZM1, or 1 μM FPS-ZM1 for 48 h. The relative cell numbers were determined by WST-1 cell proliferation assay. This experiment was replicated four times and the mean ± standard deviations (SDs) are shown in the plot.