Substrate surface temperature (circles and trend line) and humidity (triangles) gradients as a function of the distance from the warmest end of the apparatus. At the five equidistant locations receiving iButtons and hygrometers, average temperature ranged from 12.0 to 33.4 °C (±0.36 and 0.28 °C SE), whereas average humidity ranged from 84.1% to 90.7% (±0.65% SE). Temperature declined significantly as distance from the warmest end of the apparatus increased (χ2 = 408.56, df=1, p ≤ 0.001), but, there was no significant change in humidity across this temperature gradient (χ2 = 0.16, df=1, p = 0.69) nor was there a significant impact of location on humidity (χ2 = 0.01, df = 1, p=0.91). Points indicate means ± 1 standard error of seven replicates.