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. 2016 Sep 6;11(9):e0161596. doi: 10.1371/journal.pone.0161596

Fig 1. Generation of recombinant viruses and molecular constructs pNL4.3ΔEnv and pNL4.3ΔEC.

Fig 1

A and B: The molecular constructs were based on the pNL4.3 infectious clone deleted of the entire Env (gp160, ΔEnv) (A) or of the Env gp120+gp41 ectodomain (gp140, ΔEnvEC) (B) by inverse PCR. The deleted portion is replaced by the AfeI restriction site for linearization prior to transfection. C: Infectious viral particles expressing patient-derived Env or EnvEC sequences were generated by co-transfecting HEK 293T cells with the linearized pNL4.3ΔEnv or pNL4.3ΔEC backbones and a PCR amplicon spanning the target region (gp160 (Env) or gp140 (EnvEC) respectively).