Fig 1. Pneumococcus induced IκBζ expression in monocytes.

(A) Human monocytes (10⁶ cells/ml) or (B) HBECs (5X10⁴ cells/33 mm²) were infected with pneumococcus strain D39 at MOIs 0.1 and 1.0 for 3 h. LPS (1μg/ml) and rIL-1β (10ng/ml) were used as positive controls (+Ctr) for monocytes and HBECs respectively. (C) Densitometry graph for IκBζ expression normalized to house-keeping proteins HSP90 or β-actin in monocytes from blot (A) and in HBECs from blot (B). (D) Human monocytes (10⁶ cells/ml) were infected with D39 at MOI 0.1 for different time points through 24 h or (E) with different MOIs of D39 for 3 and 6h. Cell extracts were analyzed using immunoblotting with anti-IκBζ antiserum, HSP90 antibody and β-actin antibody. The immunoblots represent 3 independent experiments. NT stands for not treated. Vertical lines have been inserted to indicate a repositioned gel lane.