Fig 2. Effect of hns deletion on sslE transcription in UTI89 and EC958 at 22°C and 37°C via qRT-PCR analysis.

Relative fold-difference of sslE transcript levels of UTI89 and EC958 with their respective hns mutant and complemented strains grown at (A) 22°C (black bars) and (B) 37°C (grey bars). All mRNA levels were calculated relative to the level of UTI89 sslE mRNA at the respective temperature. The data was obtained from three independent experiments; error bars indicate standard deviation. Statistical analysis was performed using an unpaired, two-tailed t-test.