| E. coli K12 strains |
|
|
| MG1655 |
K-12 reference strain |
[42, 43] |
| BL21 (DE3) |
F–ompT hsdSB(rB–, mB–) gal dcm (DE3) |
Stratagene |
| Clinical UPEC collection |
18 strains randomly selected from a collection of isolates from patients with urosepsis |
This study |
| ExPEC strains |
|
|
| 536 |
UPEC pyelonephritis isolate |
[5, 44] |
| 536fis
|
536fis::kan; Kanr
|
This study |
| 536fis(pFis) |
536fis::kan + pFis; Kanr Cmr
|
This study |
| EC958 |
ST131 UPEC isolate |
[45–47] |
| EC958hns
|
EC958hns::cm; Cmr
|
This study |
| EC958hns(pHNS) |
EC958hns pHNS; Cmr
|
This study |
| EC958fis
|
EC958fis::cm; Cmr
|
This study |
| EC958fis(pFis) |
EC958fis pFis; Cmr
|
This study |
| IHE3034 |
NMEC isolate |
[20, 48] |
| IHE3034sslE
|
IHE3034sslE::kan; Kanr
|
This study |
| IHE3034hns
|
IHE3034hns::kan; Kanr
|
This study |
| IHE3034hns(pHNS) |
IHE3034hns::kan pHNS; Kanr Ampr
|
This study |
| IHE3034fis
|
IHE3034fis::kan; Kanr
|
This study |
| IHE3034fis(pFis) |
IHE3034fis::kan pFis; Kanr Cmr
|
This study |
| UTI89 |
UPEC cystitis isolate |
[49, 50] |
| UTI89sslE
|
UTI89sslE::kan; Kanr
|
This study |
| UTI89hns
|
UTI89hns::kan; Kanr
|
This study |
| UTI89hns(pHNS) |
UTI89hns::kan pHNS; Kanr Ampr
|
This study |
| UTI89fis
|
UTI89fis::kan; Kanr
|
This study |
| UTI89fis(pFis) |
UTI89fis::kan pFis; Kanr Cmr
|
This study |
| UTI89typA
|
UTI89typA::kan; Kanr
|
This study |
| UTI89typA(pTypA) |
UTI89typA::kan pTypA; Kanr Cmr
|
This study |
| UTI89lacI-Z
|
UTI89lacI-Z::gfp
|
This study |
| UTI89lacI-Z sslE::lacZ
|
UTI89lacI-Z::gfp sslE::lacZ
|
This study |
| UTI89lacI-Z fis
|
UTI89lacI-Z::gfp fis::kan; Kanr
|
This study |
| Plasmids |
|
|
| pKD3 |
Template plasmid for cm gene amplification |
[51] |
| pKD4 |
Template plasmid for kan gene amplification |
[51] |
| pKD46 |
λ-red recombinase expressing plasmid |
[51] |
| pCP20 |
FLP expressing plasmid |
[52] |
| pMCSG7 |
Ligation-independent 6xHis tag cloning vector |
[53] |
| pHNS |
pBR322 cloned with hns gene |
[35] |
| pSU2718 |
pACYC184-derived cloning plasmid |
[54] |
| pHNS-cm |
pSU2718 cloned with hns gene |
This study |
| pTW26 |
pSU2718 modified with a xhoI site inserted at original HincII site |
This study |
| pFis |
dusB-fis operon cloned in pTW26 |
This study |
| pTypA |
typA gene cloned in pTW26 |
This study |
| pQF50 |
Promoterless lacZ reporter plasmid |
[55] |
| pQF50-sslE
|
sslE promoter region cloned in pQF50 |
This study |
| pQF50-sslEM1 |
pQF50-sslE with 2 nucleotide mutations in F1 |
This study |
| pQF50-sslEM2 |
pQF50-sslE with 3 nucleotide mutations in F2 |
This study |
| pQF50-sslEM3 |
pQF50-sslE with 5 nucleotide mutations in F1 and F2 |
This study |
