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. 2016 Sep 6;11(9):e0161704. doi: 10.1371/journal.pone.0161704

Fig 3. Effect of procyanidins, EC and C3G on phosphorylation of PI3K and Akt in skeletal muscle of mice.

Fig 3

ICR mice were treated as described in Fig 2. Tissue lysate of skeletal muscle was prepared 60 min after the administration. Then, these lysate was subjected to immunoblotting analysis to determine (A) p-PI3K and PI3K; and (B) p-Akt serine 473 and threonine 308 and Akt. Each panel shows a typical result from six animals. The density of each band was analyzed and shown in the bottom panel. Values are means ± SE (n = 6). Different superscripted letters indicate significant differences between the groups (p <0.05; Tukey-Kramer multiple comparison test).