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. 2016 Sep 1;24(5):529–535. doi: 10.4062/biomolther.2016.011

Fig. 3.

Fig. 3.

Lobaric acid antagonized SLIGKV-NH2 effects on differentiation protein expression. Effect of SLIGKV-NH2 on involucrin, transglutamase 1 (TGM1), and filaggrin in HaCaT keratinocytes (A–B) and normal human epidermal keratinocytes (NHEKs) (C–D) by Western blot. HaCaT keratinocytes were treated with 100 μM SLIGKV-NH2 at indicated calcium concentration for 72 hrs. SLIGKV-NH2 downregulated involucrin at 0.4 mM calcium (A). Lobaric acid (0.1, 0.5, 2 μM) was used to treat cells before SLIGKV-NH2 treatment. Lobaric acid antagonized SLIGKV-NH2 effects in a non-dose-dependent manner. Positive control was 2.8 mM calcium and G protein inhibitor PTX (0.5 μg/ml) to induce differentiation and inhibit PAR2. PTX negative control was PTX-B (0.5 μg/ml) (B). SLIGKV-NH2 upregulated involucrin, TGM1 and filaggrin and lobaric acid antagonized SLIGKV-NH2 in NHKS (C–D). Positive control was 1.5 mM calcium to induce differentiation (C). Filaggrin was increased by SLIGKV-NH2 treatment and lobaric acid normalized the expression of filaggrin at 72 hours (D).