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Time-of-addition effect of norwogonin, oroxylin A, and mosloflavone on EV71 replication in Vero cells. Each compound (50 μg/mL) was added either before (−1 h), during (0 h), or after (1, 2, 4, 6, and 8 h) virus infection. After 2 days, the inhibition was evaluated by SRB method and expressed as the inhibition rate. Each value is the result of mean ± SD. of three independent experiments. **p<0.001; ***p<0.0001 using one-way ANOVA with Tukey’s post hoc test.
