Skip to main content
. 2016 Sep 1;24(5):552–558. doi: 10.4062/biomolther.2015.200

Fig. 4.

Fig. 4.

The effect of norwogonin, oroxylin and mosloflavone on EV71 replication. (A) Real-time PCR analyses were performed to determine the effect of norwogonin, oroxylin A and mosloflavone on EV71 NCR gene expression levels. Replication of EV71 from Vero cells at 48 h after infection by EV71 in the presence of 50 μg/mL norwogonin, oroxylin A and mosloflavone was determined by real-time PCR. Vehicle (0.1% DMSO)-treated cells without EV71 infection was used as control. (B) Western blot analyses were performed to determine the effect of norwogonin, oroxylin A, mosloflavone, and ribavirin on the production of EV71 VP2 proteins. The reduction in protein expression of EV71 VP2 was identified after treatment with 50 μg/mL norwogonin, oroxylin A, mosloflavone, and ribavirin for 48 h. α-tubulin was used as a loading control for each set of samples. **p<0.001 using one-way ANOVA with Tukey’s post hoc test.