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. Author manuscript; available in PMC: 2016 Sep 6.
Published in final edited form as: Dev Biol. 2016 Feb 2;411(2):217–230. doi: 10.1016/j.ydbio.2016.01.039

Fig. 3.

Fig. 3

Cell type-specific RNAi-mediated knockdown of ACF subunits in oogenesis causes apoptotic and packaging phenotypes. (A) Schematic drawing of early Drosophila oogenesis. Color code for cell type-specific expression pattern of different driver lines: MTD-Gal4 (germline cells; light and dark orange), matα4-Gal4 (germline cells stage 1 onwards, dark orange), c587-Gal4 (somatic escort and early follicle cells, purple) and traffic jam-Gal4 (somatic follicle cells, green). (B–I) Immunofluorescence images showing cell type-specific ACF1 knockdown in oogenesis. Germarium, ovariole or egg chamber with staining of ACF1 (green), Orb (red) and DNA (blue) are shown for: (B) UAS-shGFP≪MTD-Gal4 (C), UAS-shAcf1≪MTD-Gal4 (D) UAS-shGFP≪matα4-Gal4, (E) UAS-shAcf1≪matα4-Gal4, (F) UAS-shGFP≪c587-Gal4, (G) UAS-shAcf1≪c587-Gal4, (H) UAS-shGFP≪traffic jam-Gal4 and (G) UAS-shGFP≪traffic jam-Gal4. Arrows indicate oocyte nuclei. White dashed lines indicate escort cells. Color-coded square represents specific cell-type expression pattern in Fig. 3A. Scale bar: 10 μm. (J) Quantification of apoptotic and packaging phenotypes. The data show mean values in percent with SD of three biological replicates. N represents the total number of scored egg chambers stage 3–10. All analyzed ovary samples of shIswi with MTD-Gal4 (n=14) showed an agametic phenotype but detailed oogenesis phenotypes were not determined (ND) further. Two-tailed Student’s t-test was used. * represents a p-Value of <0.05 and ** <0.01. n.s. represents not significant.