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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2016 May 3;163:147–156. doi: 10.1016/j.jsbmb.2016.04.022

Figure 6. Summary of the inhibitory effects of 1,25(OH)2D on glutamine metabolism in MCF10A-ras.

Figure 6

Intracellular glutamine and its related metabolites (in red font color) levels were decreased by 1,25(OH)2D in MCF10A-ras cells. 1,25(OH)2D inhibited fractional glutamine and glutamate flux into the TCA cycle, as well as glutamine flux into α-ketoglutarate shown by red downward arrows. 1,25(OH)2D significantly decreased SLC1A5 mediated glutamine uptake into the cells, as well as the mRNA and protein abundance of SLC1A5. Abbreviations: GLS, glutaminase; GS, glutamine synthetase; PSAT, phosphoserine aminotransferase; GPT, glutamate-pyruvate transaminase; GOT, glutamicoxaloacetic transaminase; GDH, glutamate dehydrogenase.