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WecA-catalyzed biosynthesis of C50-P-P-Glucosamine-C6-FITC (3).
A: Effect of the phase transfer catalyst CHAPS. B: Effect of concentrations of MgCl2. C: Effect of concentrations of P-60 obtained from M. smegmatis. D: Time-course experiments of biosynthesis of 3 with different sources of membrane fractions. E: Effect of additives (ATP and EDTA). F: Time-course experiments of biosynthesis of 3 in the absence of exogenous C50-P.
