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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Yeast. 2016 Jun 29;33(9):507–517. doi: 10.1002/yea.3172

Figure 1. Characterization of SPAC688.07cΔ (rng10Δ).

Figure 1

A) Differential interference contrast (DIC) images of wildtype and rng10Δ cells, illustrating the cell lysis phenotype. Quantitation of cell lysis is included to the right (n=100 for each data point, the average from three replicates and standard deviation are plotted). Scale bar, 5 μm. B) Montages of live cell images of rng10-GFP sid4-RFP cells. Left panel emphasizes the arrival time of Rng10 to the division site; right panel shows Rng10 localization during contractile ring constriction. Scale bar for both right and left panels, 5 μm. C) List of proteins that localize to the cell division site identified by LC-MS of a Rng10-GFP GFP-trap experiment. TSC = total spectral counts. D) Negative genetic interactions of rng10Δ with mutations in glucan synthase genes (cps1/bgs1 and bgs4/cwg1) and septation regulatory genes (rgf3 and sid2). 10-fold dilutions of the indicated strains were spotted on YE plates and incubated for 2–3 days at the indicated temperatures.