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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Stem Cell Res. 2016 May 22;17(1):122–129. doi: 10.1016/j.scr.2016.05.012

Fig. 2.

Fig. 2

Analysis of colony area and intensity over time. (A–C) Each trajectory represents the change in area of an individual colony over time for colonies from preparations 1–3, respectively. The colors of the lines indicate average intensity of GFP fluorescence as indicated by the color bar scale. Trajectories reflect the change in the area of a colony and the average intensity of those colonies during sequential frames between colony merger events. Each pixel area = 0.394 μm2 and each frame in the time series was acquired at 45 min intervals.