Fig. 5.

(A–C) Simplex triangles for preparation 1, 2 and 3 respectively. The mean ‘homogeneous’, ‘heterogeneous’, and ‘non-expressing’ intensity and textural character of a colony over time is indicated by its location in the triangle. The upper left vertex corresponds to ‘homogeneous’; colonies, the upper right vertex corresponds to ‘heterogeneous’ colonies, and the lower vertex corresponds to ‘non-expressing’ colonies. The color of the marker relates to the scale bar on the right and indicates the magnitude of the temporal fluctuations of Oct4 character exhibited by the colony. The size of the marker indicates the colony area (i.e., the average number of hexagons associated with the colony over its lifetime) as shown in the scale in the lower right hand corner of (A). The minimum temporal fluctuation scores for homogeneous and heterogeneous colonies that appear stable are 0.05 and 0.1, respectively (as determined from Supplemental Movies 2–4 and 5–7).