Figure 1.

Effects of senolytic treatment on senescent cell burden, DNA damage, vasomotor function, nitric oxide signaling, calcification, and osteogenic signaling in chronologically aged mice. Chronic treatment with Dasatinib + Quercetin reduced senescent cell burden (A–B) and DNA damage (C) in aorta (Panel A pseudocolor legend for high magnification lower panels: DAPI/nuclei (blue), γH2A.X (green), telomeres (red). Low magnification micrographs in upper images are provided as an anatomic frame of reference/origin). White squares mark regions that are magnified in subsequent micrographs, and telomere‐associated foci are numbered in the bottom panels. Chronic senescent cell clearance improved vascular relaxation to acetylcholine (D) independently from changes in sodium nitroprusside (E) and significantly increased p‐VASP ²³⁹ levels (F). Chronic senolytic treatment with Dasatinib + Quercetin also tended to reduce vascular calcification (G), which was associated with modest reductions in osterix immunofluorescence (H). In all panels, asterisk denotes p < 0.05.